J. Pérez, M. Herrmann, D. Gradl, and A. Naber

Proc. SPIE, Vol. 7571, 757118 (2010)

Abstract:

The application of Fluorescence Correlation Spectroscopy (FCS) to Near-field Scanning Optical Microscopy on diffusing molecules in solution is presented. The ultra small excitation volume in such a technique allows measurements with fluorophore concentrations of up to 10μM, making FCS in biological environment at nearly native conditions possible.